Anti-TOM1L1 Rabbit Polyclonal Antibody for WB, ELISA - O75674

Core Product Specifications and Parameters

Biological Background: TOM1L1 Function and Localization

• TOM1L1 (TOM1-like protein 1), also known as Src-activating and signaling molecule protein (SRCASM) or Target of Myb-like protein 1, is encoded by the TOM1L1 gene in humans. Alternative splicing produces multiple isoforms.
• It functions as a probable adapter protein involved in intracellular signaling pathways, linking upstream signals to downstream effectors.
• When phosphorylated, TOM1L1 interacts with the SH2 and SH3 domains of various signaling proteins, facilitating signal complex assembly.
• It may promote FYN kinase activation, possibly by disrupting intramolecular SH3-dependent autoinhibitory interactions.
• TOM1L1 localizes predominantly to the Golgi apparatus (Golgi stack), endosome membrane, cytoplasm, and cell membrane, consistent with a role in vesicular trafficking and signaling.
• Post-translational phosphorylation is critical for its function, enabling interactions with SH2 domain-containing partners.
• Keywords from UniProt include: alternative splicing, cytoplasm, endosome, Golgi apparatus, membrane, phosphoprotein, protein transport, SH3-binding, and transport.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 250–350 of human TOM1L1 (NP_005477.2); consider verifying sequence conservation in mouse and rat to confirm expected cross-reactivity.
• For western blot (WB), use whole-cell lysates from human, mouse, or rat cell lines; the predicted molecular weight is 53 kDa, but additional bands may appear due to alternative splicing or post-translational modifications.
• ELISA applications may require optimization of coating antigen and antibody titer; consider performing a checkerboard titration to establish suitable conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a comprehensive one-stop sourcing solution for IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For TOM1L1 and related signaling adapter targets, CamelBio offers validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whether you need routine antibodies or specialized reagents for signaling pathway research, CamelBio streamlines procurement and accelerates your development pipeline.