Anti-TOM1 Rabbit Polyclonal Antibody for WB and ELISA - O60784

Core Product Specifications and Parameters

Biological Background: TOM1 Function and Localization

• TOM1 (Target of Myb1) is an adapter protein involved in intracellular membrane trafficking of ubiquitinated proteins, contributing to autophagy, ubiquitination-dependent signaling, and receptor recycling pathways. Related references: PMID:14563850, PMID:15047686, PMID:23023224, PMID:31371777
• It binds polyubiquitinated proteins via its GAT domain and forms a complex with TOLLIP on early endosomes, facilitating the recruitment of ubiquitin-conjugated cargo to clathrin. Related references: PMID:14563850, PMID:15047686, PMID:15657082
• Acts as a MYO6/myosin VI adapter, targeting MYO6 to endocytic structures and promoting autophagosomal delivery and maturation. Related references: PMID:23023224, PMID:31371777
• Functions as a phosphatidylinositol 5-phosphate (PtdIns(5)P) effector, regulating endosomal maturation; PtdIns(5)P-dependent recruitment to signaling endosomes may block maturation. Related references: PMID:25588840
• Modulates TOLLIP binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) through binding competition, influencing TOLLIP release from endosomal membranes. Related references: PMID:26320582
• Negatively regulates Toll-like receptor (TLR) signaling and participates in immune receptor recycling. Related references: PMID:15047686, PMID:26320582
• Localizes primarily to the cytoplasm and endosomal membranes (early endosomes). Widely expressed, with high levels in skeletal muscle, heart, placenta, and liver. Undergoes acetylation and phosphorylation; alternative splicing produces multiple isoforms; associated with disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 314–493) of human TOM1; this region may be well-conserved across species, consistent with observed cross-reactivity with mouse and rat.
• For Western blotting, the predicted molecular weight is approximately 54 kDa. Consider using appropriate positive controls (e.g., tissues with high TOM1 expression like skeletal muscle or placenta) and validate band specificity.
• For ELISA applications, consider employing this antibody as a capture or detection reagent in sandwich immunoassays; pair with a validated TOM1 antibody to optimize performance. Validate in the relevant sample matrix.
• Given TOM1's role in endosomal trafficking, consider co-localization studies or additional validation in functional assays if investigating autophagy or TLR signaling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies such as this anti-TOM1 antibody for autophagy and trafficking studies. We offer one-stop access to antibody pairs, optimized bulk reagents, and custom sourcing for rare targets, supporting your workflow from discovery to clinical application.