Anti-TOLLIP Rabbit Monoclonal Antibody (KD Validated) for WB, IHC-P, ELISA - Q9H0E2

Core Product Specifications and Parameters

Biological Background: TOLLIP Function and Localization

• TOLLIP is a key component of the IL-1 and Toll-like receptor signaling pathway, inhibiting cell activation by microbial products, and recruits IRAK1 to the receptor complex, suppressing its phosphorylation and kinase activity. Related references: PMID:10854325, PMID:11751856
• It functions as a ubiquitin-ATG8 family adapter, linking the ubiquitin pathway to autophagy and mediating autophagic clearance of ubiquitin conjugates and cytotoxic polyQ protein aggregates. Related references: PMID:25042851
• In complex with TOM1, TOLLIP recruits ubiquitin-conjugated proteins onto early endosomes. Related references: PMID:15047686
• TOLLIP binds to phosphatidylinositol 3-phosphate (PtdIns(3)P), which contributes to its endosomal localization. Related references: PMID:26320582
• The protein is primarily localized in the cytoplasm and on early endosomes.
• Post-translational modifications include phosphorylation and acetylation, and multiple isoforms exist due to alternative splicing.
• TOLLIP plays a critical role in innate immunity and inflammatory response, as highlighted by its keyword associations.

Experimental Guidance and Technical Tips

• As the immunogen covers amino acids 1-274, this antibody may recognize full-length TOLLIP and potentially N-terminal isoforms; validate in your system.
• For Western blot, the antibody is KD validated, but confirm specificity and sensitivity under your experimental conditions.
• In IHC-P, consider validating in tissues with known TOLLIP expression, such as lymphoid organs or inflamed tissues.
• For ELISA, the recombinant fusion protein used as immunogen could serve as a positive control; optimize coating and detection conditions.
• Cross-reactivity with mouse and rat suggests conservation of the epitope; however, validation in those species' samples is recommended.

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CamelBio is your comprehensive partner for IVD raw-material sourcing, offering a complete range of products and services to support diagnostic development from concept to clinic. Our portfolio includes validated antibodies like this anti-TOLLIP monoclonal antibody, essential for studying innate immunity and autophagy pathways, as well as antibody pairs, bulk reagents, and custom sourcing for rare targets. Streamline your procurement with our one-stop solution.