Anti-TOLLIP Polyclonal Antibody for WB, ELISA - Q9H0E2

Core Product Specifications and Parameters

Biological Background: TOLLIP Function and Localization

• TOLLIP is a critical negative regulator of IL-1 and Toll-like receptor (TLR) signaling pathways; it recruits IRAK1 to the IL-1 receptor complex and directly inhibits IRAK1 phosphorylation and kinase activity, thereby suppressing cell activation by microbial products. Related references: PMID:10854325 PMID:11751856
• Acting as a ubiquitin-ATG8 family adapter, TOLLIP links the ubiquitin pathway to autophagy and mediates autophagic clearance of ubiquitin conjugates, including cytotoxic polyQ protein aggregates, playing an essential role in selective autophagy. Related references: PMID:25042851
• In a complex with TOM1, TOLLIP recruits ubiquitin-conjugated proteins onto early endosomes, facilitating their trafficking and degradation. Related references: PMID:15047686
• TOLLIP interacts with phosphatidylinositol 3-phosphate (PtdIns(3)P), a phosphoinositide enriched on early endosomes, which likely contributes to its endosomal localization and function. Related references: PMID:26320582
• Subcellularly, TOLLIP predominantly localizes to the cytoplasm and early endosomes, consistent with its roles in signaling and endosomal trafficking.
• The protein undergoes acetylation and phosphorylation, and alternative splicing generates multiple isoforms, expanding its regulatory potential in innate immunity and inflammatory responses.

Experimental Guidance and Technical Tips

• The immunogen used to raise this antibody corresponds to the full-length human TOLLIP (amino acids 1–274), which shares high sequence identity with mouse and rat orthologs; therefore, broad cross-reactivity is expected, but verification in the intended species and sample type is recommended.
• This polyclonal antibody is suitable for Western blot (WB) and ELISA. For WB, consider using appropriate positive (e.g., human THP-1, mouse RAW264.7) and negative controls, and validate the observed band size (~30 kDa) against the predicted molecular weight.
• Given TOLLIP's involvement in multiple signaling contexts, experimental conditions (e.g., stimulation with IL-1β or TLR ligands) may influence expression levels; optimise detection protocols accordingly.

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CamelBio serves as a comprehensive IVD raw material partner, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. Whether you need validated antibody pairs, bulk ancillary reagents, or rare target sourcing for innate immunity and autophagy research, we provide reliable one-stop access to critical components like this TOLLIP polyclonal antibody, streamlining your development pipeline with expert technical and procurement support.