Anti-TOB2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14106

Core Product Specifications and Parameters

Biological Background: TOB2 Function and Localization

• Protein identity: TOB2 (Transducer of erbB-2 2), also designated as TOB4, TOBL, APRO5, and TROB2, is encoded by the TOB2 gene with synonyms KIAA1663, TOB4, TROB2.
• Core function: Functions as an anti-proliferative protein, inhibiting cell cycle progression from the G0/G1 to S phases.
• Subcellular localization: Localized primarily in the cytoplasm.
• Tissue expression: Expressed ubiquitously across tissues.
• Post-translational modifications: Identified as a phosphoprotein (based on proteomics data), indicating potential regulation by phosphorylation.
• Molecular weight: The canonical isoform has a calculated molecular weight of approximately 36.6 kDa (UniProt: 36632 Da).
• Keywords: The protein is subject to alternative splicing and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 70 amino acids; the antibody may recognize all isoforms sharing this region, but isoforms lacking it might not be detected.
• For WB, the predicted molecular weight is 37 kDa. However, phosphorylation or alternative splicing may cause band shifts or multiple bands. Validate in the relevant sample system.
• For IHC-P, perform antigen retrieval as needed and validate in positive and negative control tissues. Ubiquitous expression suggests many tissues as positive controls; consider including a TOB2 knockout as negative control.
• For ELISA, consider pairing with a capture antibody for sandwich detection or use the antibody directly to detect TOB2 in cell lysates.
• Since the antibody cross-reacts with mouse and rat, it is suitable for cross-species studies, but always verify specificity in the chosen model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For anti-TOB2 research in cell cycle and oncology, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development needs.