Anti-TNRC6A Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NDV7

Core Product Specifications and Parameters

Biological Background: TNRC6A Function and Localization

• TNRC6A, also known as GW182, TNRC6, CAGH26, or GW1, is a 210 kDa protein encoded by the TNRC6A gene on chromosome 8. It is a key component of the RNA-induced silencing complex (RISC).
• Functions in RNA-mediated gene silencing by microRNAs (miRNAs) and siRNAs. It is required for miRNA-dependent translational repression and siRNA-dependent endonucleolytic cleavage.
• Acts as a scaffolding protein, associating with argonaute proteins bound to partially complementary mRNAs, and recruiting the CCR4-NOT and PAN deadenylase complexes to facilitate mRNA decay.
• Localizes predominantly to the cytoplasm, specifically enriched in processing bodies (P-bodies), which are sites of mRNA degradation and storage.
• Ubiquitously expressed across human tissues, reflecting its fundamental role in post-transcriptional gene regulation.
• Post-translationally phosphorylated and undergoes alternative splicing, producing multiple isoforms that may modulate its activity or interactions.
• Contains RNA-binding domains and a coiled-coil region; involved in host-virus interactions and linked to epilepsy through genetic association studies.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 950–1050 of human TNRC6A (NP_055309.2). This region may be conserved across species, supporting cross-reactivity with mouse and rat.
• For western blot (WB), consider using whole-cell lysates from human, mouse, or rat cell lines, as TNRC6A is ubiquitously expressed. The predicted molecular weight is ~210 kDa; ensure adequate gel resolution and transfer conditions for high-molecular-weight proteins.
• In immunofluorescence (IF) or immunocytochemistry (ICC), cytoplasmic and P-body localization is expected. Co-staining with P-body markers (e.g., DCP1A) may confirm specificity.
• For ELISA applications, validate the antibody using recombinant TNRC6A or peptide blocking assays to confirm binding affinity and specificity.
• As a polyclonal antibody, lot-to-lot variability may occur. Include appropriate controls and optimize dilution factors for each experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-TNRC6A polyclonal antibody is a valuable tool for investigating RNA silencing and RISC-related pathways. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and targeted sourcing for rare raw materials, helping you streamline your project from concept to clinic.