Anti-TNR Polyclonal Antibody for WB, ELISA - Q92752

Core Product Specifications and Parameters

Biological Background: TNR Function and Localization

• Tenascin-R (TNR), also known as janusin or restrictin, is a secreted neural extracellular matrix protein belonging to the tenascin family.
• It mediates both adhesive and anti-adhesive interactions, capable of evoking stable adhesion and differentiation or repulsion and inhibition of neurite outgrowth depending on the cellular context.
• Binds to cell surface gangliosides to inhibit integrin-mediated cell adhesion and FAK phosphorylation; interaction with sulfatides promotes oligodendrocyte adhesion and differentiation.
• Interaction with contactin-1 (CNTN1) induces neuronal repulsion and inhibits neurite outgrowth; association with sodium channel subunit beta-2 (SCN2B) may cluster and regulate sodium channels at nodes of Ranvier.
• TNR-linked chondroitin sulfate glycosaminoglycans mediate binding to fibronectin (FN1), contributing to the inhibition of cell adhesion and neurite outgrowth.
• Localized to the extracellular space and matrix, with expression restricted to the brain.
• Subject to extensive post-translational modifications including glycosylation, phosphorylation, and proteoglycan attachment; contains multiple EGF-like and fibronectin type-III repeat domains.
• Alternative splicing generates multiple isoforms; UniProtKB keywords indicate relevance to disease variants and proteomic identification.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1059–1358 within the C-terminal fibronectin type-III repeats, a region conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blot (WB), the predicted molecular weight is approximately 150 kDa; note that isoforms and post-translational modifications (e.g., glycosylation, proteoglycan attachment) may produce additional bands.
• For ELISA, the antibody can be used to detect recombinant or native TNR; validate performance in the relevant sample matrix (e.g., brain tissue lysates or extracellular matrix preparations).
• Consider performing a titration experiment to determine the optimal working concentration for your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop partner for IVD raw material sourcing, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. Our supply capabilities include validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. The anti-TNR polyclonal antibody is ideally suited for neuroscience and extracellular matrix research applications, and we invite you to explore our full portfolio of neural ECM-related reagents.