Anti-TNPO1 Polyclonal Antibody for WB, ELISA - Q92973

Core Product Specifications and Parameters

Biological Background: Transportin-1 (TNPO1) Function and Localization

• Transportin-1 (importin beta-2 / KPNB2 / MIP) is a member of the karyopherin beta family, involved in nucleocytoplasmic transport.
• It functions as a nuclear import receptor for M9-containing cargo proteins, such as hnRNP A1 and A2, histones H2A, H2B, H3, H4, and ribosomal proteins.
• The protein localizes to both the cytoplasm and nucleus, shuttling between these compartments in a Ran-dependent manner.
• Mediates nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5, and SRP19. Related references: PMID:11682607
• Mediates nuclear import of ADAR1 isoforms in a RanGTP-dependent fashion. Related references: PMID:19124606 PMID:24753571
• Serves as the main import receptor for BAP1, a component of the PR-DUB complex, with redundancy provided by KPNA1 and KPNA2. Related references: PMID:35446349
• Post-translational modifications include acetylation, and alternative splicing generates multiple isoforms.
• In HIV-1 infection, TNPO1 mediates nuclear import of the viral Rev protein, facilitating viral replication.

Experimental Guidance and Technical Tips

• The immunogen consists of a synthetic peptide from the C-terminal region (aa 790–890), likely recognizing full-length TNPO1; consider validating specificity in lysates from cells with known TNPO1 expression.
• For Western blot (WB), the predicted molecular weight is approximately 102 kDa; a 6–8% SDS-PAGE gel may be optimal for resolution.
• In ELISA applications, the peptide immunogen can serve as a positive control for assay optimization.
• Cross-reactivity is reported with human, mouse, and rat TNPO1; confirm reactivity in the relevant species by preliminary testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, providing diagnostic manufacturers and research laboratories with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets. For nuclear transport and importin pathway studies, we offer a range of antibodies against key transporters like TNPO1, ensuring reliable supply from discovery through assay development. Explore our catalog to secure consistent, high-quality materials for your diagnostic needs.