Anti-TNNT1 Polyclonal Antibody for WB, ELISA - P13805

Core Product Specifications and Parameters

Biological Background: TNNT1 Function and Localization

• Troponin T, slow skeletal muscle (TNNT1) is the tropomyosin-binding subunit of the troponin complex specific to slow-twitch skeletal muscle fibers.
• It confers calcium-sensitivity to striated muscle actomyosin ATPase activity, playing a central role in muscle contraction regulation.
• Encoded by the TNNT1 gene, this protein generates multiple isoforms through alternative splicing.
• Localizes to the thin filament of the sarcomere in slow skeletal muscle cells.
• Post-translational modifications include phosphorylation, which may modulate contractile function.
• Mutations in TNNT1 are associated with nemaline myopathy, a congenital muscle disorder characterized by muscle weakness and structural anomalies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-262 of human TNNT1; when designing similar assays, consider that epitopes are likely present in this region.
• The antibody is applicable for Western blot (WB) and ELISA; for other applications like IHC or IF, validation in the relevant sample system is recommended.
• A predicted band may be observed around 33 kDa in Western blot; however, actual migration can vary depending on the isoform and sample type.
• Cross-reactivity is reported for human samples; testing in other species may require sequence homology analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to validated IVD raw materials, including polyclonal antibodies like this anti-TNNT1 antibody, for diagnostic manufacturers and researchers. With expertise in muscle-related targets and rare disease markers, we support your project from concept to clinic by supplying bulk ancillary reagents, matched antibody pairs, and custom sourcing solutions. Let us bridge your procurement needs in cardiac and skeletal muscle research.