Anti-TNNI3K Polyclonal Antibody for WB, ELISA - Q59H18

Core Product Specifications and Parameters

Biological Background: TNNI3K Function and Localization

• TNNI3K, also known as cardiac ankyrin repeat kinase (CARK) or TNNI3-interacting kinase, is a serine/threonine-protein kinase encoded by the TNNI3K gene.
• It is characterized by an ANK repeat domain and a coiled-coil region, typical of proteins involved in protein-protein interactions and signal transduction.
• The kinase domain binds ATP and requires magnesium as a cofactor for catalytic activity.
• TNNI3K is highly and specifically expressed in both adult and fetal heart tissue, indicating a critical role in cardiac biology.
• It localizes to both the nucleus and the cytoplasm, suggesting multifaceted functions in gene regulation and cytoplasmic signaling.
• Functional studies suggest it may play a role in cardiac physiology, and genetic variants in TNNI3K have been linked to cardiomyopathy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-75 of human TNNI3K (NP_057062.1), covering the N-terminal region. This region may be conserved across isoforms; verify isoform specificity if required.
• For Western blot, the predicted molecular weight is approximately 93kDa; however, post-translational modifications may cause shifts. Use appropriate positive controls from heart tissue or cell lines expressing TNNI3K.
• In ELISA applications, consider pairing with a matched detection antibody and optimize coating/detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive access to IVD antibody raw materials, including polyclonal and monoclonal antibodies for cardiac biomarker targets like TNNI3K. We offer validated antibody pairs, ancillary reagents, and custom sourcing for rare targets, helping accelerate your assay development from concept to clinic. Rely on CamelBio as your procurement partner for high-quality, reproducible raw materials.