Anti-TNNC2 Polyclonal Antibody for WB, IHC-P, ELISA - P02585

Core Product Specifications and Parameters

Biological Background: TNNC2 Function and Localization

• TNNC2 encodes troponin C, the calcium-binding subunit of the troponin complex in fast-twitch skeletal muscle fibers.
• Troponin C (TnC) forms a heterotrimeric complex with troponin I (TnI) and troponin T (TnT) to regulate striated muscle contraction.
• Upon calcium binding, TnC undergoes a conformational change that relieves TnI-mediated inhibition of actomyosin ATPase activity, permitting muscle contraction.
• The protein contains four EF-hand motifs, though only two are functional calcium-binding sites in skeletal muscle isoforms.
• TNNC2 is predominantly expressed in fast-twitch skeletal muscle, distinguishing it from the slow/cardiac isoform TNNC1.
• Post-translational modifications include N-terminal acetylation, which may influence protein stability and function.
• Mutations in TNNC2 have been linked to congenital myopathy and other skeletal muscle disorders.
• The crystal structure of troponin C has been solved, revealing a dumbbell-shaped molecule with N- and C-terminal globular domains connected by an alpha-helical linker.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human TNNC2 (amino acids 1–160), ensuring recognition of both native and recombinant protein.
• For Western blot, the observed band should be approximately 18 kDa under reducing conditions; consider using a low-molecular-weight marker for accurate sizing.
• For immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval) may be required for optimal staining of formalin-fixed paraffin-embedded tissues.
• When using ELISA, validate the antibody in your assay system with appropriate positive and negative controls, as performance may vary with plate coating and detection conditions.
• Cross-reactivity with mouse and rat samples has been reported; confirm species-specific band patterns in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with convenient access to high-quality IVD raw materials, technical services, and consulting—supporting your journey from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for assays targeting skeletal muscle diseases and troponin C research. For rare or custom targets, our sourcing expertise helps you secure critical raw materials efficiently.