Anti-TNNC1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P63316

Core Product Specifications and Parameters

Biological Background: TNNC1 Function and Localization

• Troponin C (TNNC1) is the calcium-binding subunit of the troponin complex, a central regulatory system of striated muscle contraction.
• It is predominantly expressed in slow skeletal and cardiac muscles.
• The troponin complex comprises three subunits: Tn-C (calcium-binding), Tn-I (inhibitory), and Tn-T (tropomyosin-binding).
• Binding of calcium to Tn-C abolishes the inhibitory action of Tn-I on actomyosin ATPase, thereby enabling muscle contraction.
• TNNC1 is associated with cardiomyopathy and disease variants, as indicated by UniProt keywords.
• Post-translational modifications of TNNC1 include acetylation and phosphorylation, which may modulate its function.
• The protein contains calcium-binding EF-hand repeats, classifying it as a metal-binding protein.
• TNNC1 has been directly sequenced and identified in proteomics studies, underscoring its relevance in muscle biology and pathology.

Experimental Guidance and Technical Tips

• For Western blot, expect a band around 18 kDa consistent with the predicted molecular weight of TNNC1. Validate specificity using knockout or knockdown controls.
• In immunofluorescence (IF-P), test staining on cardiac or slow skeletal muscle tissue sections; include a negative control to confirm signal localization.
• For ELISA, use recombinant TNNC1 protein or tissue lysates as coating antigen; optimize antibody concentration and detection conditions.
• The immunogen spans nearly the full-length protein (aa 1-160), so the antibody may recognize both native and denatured TNNC1; consider this for assay design.
• Given cross-reactivity with mouse and rat, this antibody can be used for translational studies in rodent models; verify performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and services, supporting diagnostic development from concept to clinic. For cardiac troponin research and related biomarker applications, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Contact us to source rare targets or customize reagent panels—our team bridges your procurement and R&D needs efficiently.