Anti-TNNC1 Rabbit Monoclonal Antibody for WB and ELISA - P63316

Core Product Specifications and Parameters

Biological Background: TNNC1 Function and Localization

• TNNC1 encodes troponin C, slow skeletal and cardiac muscles, a calcium-binding subunit of the troponin complex essential for striated muscle contraction.
• Troponin consists of three components: Tn-I (inhibitor of actomyosin ATPase), Tn-T (binds tropomyosin), and Tn-C; calcium binding to Tn-C relieves Tn-I inhibition, allowing actin-myosin interaction.
• TNNC1 is primarily expressed in slow skeletal and cardiac muscle tissues, where it regulates contraction in response to calcium fluxes.
• The protein is a member of the troponin gene family, which includes isoforms specific to different muscle types (e.g., TNNT1, TNNI3).
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomic studies; mutations in TNNC1 are associated with cardiomyopathy (CMD1Z, CMH13).
• Keywords from UniProt highlight roles in calcium binding, metal binding, muscle contraction, and disease involvement.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–100 of human TNNC1; the antibody is expected to recognize the N-terminal region of the protein.
• For Western blot, denaturing and reducing conditions are recommended; the predicted molecular weight is 18 kDa. Validate in appropriate muscle tissue lysates (e.g., cardiac, skeletal) from human, mouse, or rat.
• ELISA applications may benefit from using recombinant TNNC1 or peptide antigens; consider testing direct and sandwich ELISA formats.
• Cross-reactivity with mouse and rat indicates sequence conservation; however, verify specificity in your experimental system by including appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic development. This anti-TNNC1 monoclonal antibody supports cardiac and muscle research programs, and our one-stop sourcing model can also assist in securing rare targets and custom raw-material solutions. As your B2B partner, we streamline procurement from concept to clinic, helping you accelerate assay development and manufacturing.