Anti-TNKS1BP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9C0C2

Core Product Specifications and Parameters

Biological Background: TNKS1BP1 Function and Localization

• Encoded by the TNKS1BP1 gene, the 182 kDa tankyrase-1-binding protein (TAB182) is also known as KIAA1741.
• The gene undergoes alternative splicing, producing multiple transcript variants.
• TNKS1BP1 localizes to multiple subcellular compartments including the nucleus, cytoplasm, cytoskeleton, and chromosomes.
• It is a phosphoprotein and subject to post-translational modifications such as ADP-ribosylation and methylation.
• Tissue expression is detected in testis, ovary, lung, skeletal muscle, heart, prostate, and pancreas, with very low levels in brain and peripheral blood leukocytes.
• The protein has a predicted molecular weight of approximately 182 kDa, consistent with its role as a tankyrase-1 interacting scaffold protein.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 182 kDa is expected; consider using a lower percentage gel for better resolution of high-molecular-weight proteins.
• For immunohistochemistry (paraffin-embedded), antigen retrieval may be necessary; validate staining in positive control tissues such as testis or ovary where expression is high.
• The immunogen spans a region conserved across human, mouse, and rat, supporting the observed cross-reactivity; however, sequence alignment confirmation is advisable for other species.
• For ELISA applications, use recombinant protein or peptide antigens corresponding to the immunogen region as coating antigen, and optimize coating concentration and antibody dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research laboratories with reliable sourcing of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents. This anti-TNKS1BP1 antibody can be integrated into assays for chromatin biology, cytoskeleton dynamics, or cancer biomarker discovery. For custom or bulk requirements, CamelBio offers flexible supply solutions across the full development pipeline.