Anti-TNK1 Polyclonal Antibody for WB and ELISA - Q13470

Core Product Specifications and Parameters

Biological Background: TNK1 Function and Localization

• TNK1 (Non-receptor tyrosine-protein kinase TNK1, also known as CD38 negative kinase 1) is a member of the tyrosine kinase family containing an SH3 domain and ATP-binding site.
• It functions as a negative regulator of cell growth and possesses tumor suppressor properties by inhibiting the Ras-MAPK signaling pathway.
• TNK1 is involved in phospholipid signal transduction and may play a role in signaling during fetal development and in lymphohematopoietic cells.
• The protein localizes to both the cytoplasm and membrane compartments, enabling diverse signaling interactions.
• Tissue expression is broad in fetal stages (blood, brain, lung, liver, kidney) but becomes more restricted in adults, being detected mainly in prostate, testis, ovary, small intestine, colon, and hematopoietic cells.
• Post-translational modifications include phosphorylation, and the gene undergoes alternative splicing, contributing to functional diversity.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant protein covering amino acids 100–380 of human TNK1, which includes regions likely to confer specificity for the target.
• For Western blotting, consider using whole-cell lysates from human samples, as TNK1 is expressed in cytoplasm and membrane fractions; the predicted band is ~72 kDa.
• Validate the antibody’s reactivity in your specific sample matrix; cross-reactivity is reported for human only, so ensure your model system expresses TNK1.
• Due to the polyclonal nature, we recommend including positive and negative controls to monitor lot-to-lot consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. This anti-TNK1 polyclonal antibody supports kinase signaling and tumor suppression research, and we also offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare or custom targets like TNK1, our sourcing capabilities ensure you receive consistent, high-quality materials to streamline your assay development.