Anti-TNFSF13 (APRIL) Rabbit Polyclonal Antibody for WB, ELISA - O75888

Core Product Specifications and Parameters

Biological Background: TNFSF13 Function and Localization

• TNFSF13 (APRIL) is a type II transmembrane protein that can be proteolytically processed to produce a soluble cytokine, as indicated by the "Cleavage on pair of basic residues" and "Secreted" keywords.
• The soluble form functions as a cytokine binding to TNFRSF13B/TACI and TNFRSF17/BCMA, mediating signals that can regulate tumor cell growth and modulate immune cell functions.
• Alternative splicing of the TNFSF13 gene yields different isoforms, contributing to functional diversity.
• Post-translational modifications, including N-linked glycosylation and disulfide bond formation, are critical for proper folding and activity.
• High-level expression is observed in transformed cell lines and several cancer types (colon, thyroid, lymphoid), as well as in normal monocytes and macrophages, implicating it in both cancer biology and immune homeostasis.
• The 3D structure of TNFSF13 has been determined, providing insights into receptor-ligand interactions and potential therapeutic targeting.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against a recombinant protein spanning the extracellular domain (residues 105-247) of human TNFSF13, which includes receptor-binding regions. This design aids recognition of both full-length and soluble forms.
• For Western blot, a band at approximately 27 kDa (reduced) may represent the monomeric, glycosylated form; additional bands could appear due to dimerization or alternative splicing variants. Validation in the relevant sample system is recommended.
• In ELISA, this antibody can be utilized for detection of recombinant human TNFSF13 as well as endogenous protein in cell lysates or conditioned media; optimal working dilutions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials tailored for immunoassay development. Beyond this anti-TNFSF13 polyclonal antibody, we supply matched antibody pairs, recombinant proteins, and ancillary reagents to accelerate cancer and immunology research projects. Our one-stop platform streamlines sourcing from validation to bulk supply, reducing lead times and ensuring batch-to-batch consistency.