Anti-TNFRSF25 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q93038

Core Product Specifications and Parameters

Biological Background: TNFRSF25 Function and Localization

• TNFRSF25 (also known as DR3, LARD, TRAMP, APO-3) is a member of the tumor necrosis factor receptor superfamily and plays a key role in apoptosis and immune regulation.
• It serves as the receptor for TNFSF12/APO3L/TWEAK, mediating NF-kappa-B activation and apoptosis induction via interaction with the adapter protein TRADD.
• The protein exists in multiple isoforms due to alternative splicing, with the full-length form being a transmembrane receptor and others being potentially secreted.
• It is predominantly expressed in thymocytes and lymphocytes and is abundant in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen; it is also detected in the prostate.
• Key structural features include an extracellular cysteine-rich domain, a transmembrane helix, and an intracellular death domain; the protein is glycosylated and contains disulfide bonds.
• Its role in lymphocyte homeostasis suggests potential involvement in autoimmune diseases and cancer, making it a target for therapeutic research.

Experimental Guidance and Technical Tips

• The immunogen (aa 25-190) corresponds to the extracellular domain, so the antibody likely recognizes the ligand-binding region; consider using recombinant extracellular domain as a positive control.
• For Western blot, a band near 45 kDa is expected for the canonical isoform, but additional bands may appear due to glycosylation or isoform variation; validate using lysates from tissues like thymus or spleen where TNFRSF25 is highly expressed.
• In immunofluorescence, permeabilization may be required; the signal is expected at the plasma membrane, but secreted forms may also be detected.
• Cross-reactivity with mouse and rat allows for translational studies, but sequence alignment of the immunogen region is advised to ensure epitope conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials, supporting projects from target discovery to clinical application. This anti-TNFRSF25 antibody is part of our extensive portfolio for apoptosis and immunology research, where we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing assistance for rare targets. With a commitment to bridging supply gaps, CamelBio is your trusted partner for streamlining raw material procurement.