Anti-TNFRSF11B Rabbit Polyclonal Antibody for WB and ELISA - O00300

Core Product Specifications and Parameters

Biological Background: TNFRSF11B Function and Localization

• TNFRSF11B encodes osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF), a secreted glycoprotein belonging to the tumor necrosis factor receptor superfamily.
• Acts as a decoy receptor for TNFSF11/RANKL, neutralizing its function and thereby inhibiting osteoclast differentiation and activation.
• Plays a critical role in bone homeostasis; the local ratio of RANKL to OPG determines the extent of bone resorption.
• May also function as a decoy receptor for TNFSF10/TRAIL, blocking TRAIL-induced apoptosis and further inhibiting osteoclastogenesis.
• Implicated in preventing arterial calcification, suggesting a protective role in vascular biology.
• Highly expressed in adult lung, heart, kidney, liver, and bone marrow, with lower levels in brain, placenta, and skeletal muscle.
• Secreted protein containing disulfide bonds; post-translational modifications include glycosylation.

Experimental Guidance and Technical Tips

• For Western blotting, consider using a dilution series and validate with positive controls from high-expressing tissues such as bone marrow or osteoblast cultures.
• The immunogen spans amino acids 22–280 of the extracellular domain; membrane or whole-cell lysates may require optimization for detection of the secreted protein.
• For ELISA applications, pairing with a monoclonal anti-TNFRSF11B antibody may enhance sensitivity; optimization of coating and detection conditions is recommended.
• Upon receipt, aliquot and store at -20°C or lower to minimize freeze-thaw cycles, ensuring consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers researchers and diagnostic manufacturers a comprehensive sourcing solution for bone metabolism and osteoclast biology reagents. We provide validated antibody pairs, high-quality polyclonal and monoclonal antibodies, and bulk ancillary raw materials to support your assay development. Let CamelBio accelerate your project from concept to clinic with reliable one-stop procurement.