Anti-TNFRSF11A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y6Q6

Core Product Specifications and Parameters

Biological Background: TNFRSF11A Function and Localization

• TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily Member 11A), also known as RANK, ODFR, and CD265, is a receptor that binds the cytokine TNFSF11/RANKL/TRANCE/OPGL.
• It plays an essential role in RANKL-mediated osteoclastogenesis, a process critical for bone remodeling and immune regulation. Related references: PMID:9878548
• Interaction with the adaptor protein EEIG1 promotes osteoclastogenesis by facilitating the transcription of NFATC1 and activation of PLCG2.
• The receptor is involved in regulating interactions between T-cells and dendritic cells, linking it to both bone and immune system functions.
• Subcellularly, TNFRSF11A localizes to the cell membrane and membrane rafts, where it forms signaling complexes upon ligand engagement.
• Ubiquitously expressed, with notably high levels in skeletal muscle, thymus, liver, colon, small intestine, and adrenal gland.
• Post-translational modifications include disulfide bond formation, glycosylation, and phosphorylation, which regulate its signaling capacity.
• Mutations in TNFRSF11A are associated with bone disorders such as osteopetrosis and familial expansile osteolysis, and the protein is implicated in receptor-mediated signaling, metal binding, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular domain (aa 466–616), so the antibody is expected to recognize full-length and possibly some truncated isoforms; RANK lacking the extracellular ligand-binding domain may co-exist.
• For Western blotting, a specific band around 66 kDa (mature protein) should be observed; due to heavy glycosylation, the apparent molecular weight may shift upward in some cell types.
• Consider using osteoclast precursor cell lines (e.g., RAW264.7 treated with RANKL) or activated dendritic cells as positive controls for WB and IF/ICC.
• In IHC-P and IF/ICC, expect membranous and possibly raft-associated staining patterns; permeabilization may be required for optimal detection.
• Verify cross-reactivity with mouse and rat samples by sequence alignment; the targeted region shows high conservation but actual performance should be validated in your specific sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined supply chain for IVD raw materials, including this anti-TNFRSF11A antibody for osteoimmunology and bone disease research. We provide validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development from concept to clinic. Contact us to explore customized procurement solutions that accelerate your diagnostic and therapeutic projects.