Anti-TNFRSF11A Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y6Q6

Core Product Specifications and Parameters

Biological Background: TNFRSF11A Function and Localization

• TNFRSF11A, also known as RANK, CD265, and osteoclast differentiation factor receptor (ODFR), is a member of the tumor necrosis factor receptor superfamily, encoded by the TNFRSF11A gene.
• It functions as the receptor for TNFSF11/RANKL, playing an essential role in RANKL-mediated osteoclastogenesis. Related references: PMID:9878548
• Through interaction with EEIG1, it promotes osteoclastogenesis by facilitating NFATC1 transcription and PLCG2 activation (By similarity).
• It is involved in the regulation of interactions between T-cells and dendritic cells (By similarity).
• Subcellular localization includes the cell membrane and membrane rafts, with the immunogen corresponding to the intracellular C‑terminal region.
• Ubiquitously expressed with high levels in skeletal muscle, thymus, liver, colon, small intestine, and adrenal gland.
• UniProt keywords: disulfide bond, glycoprotein, metal‑binding, phosphoprotein, and sodium, reflecting its structural features and regulatory modifications.
• Alternative splicing and disease‑associated variants (e.g., deafness, osteopetrosis) have been documented.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 466‑616, the intracellular domain. For intracellular detection (IF/ICC), permeabilization is required.
• For Western blotting, use a standard 10% SDS‑PAGE gel and a loading control; the predicted molecular weight is 66kDa.
• In IHC‑P, antigen retrieval (e.g., citrate buffer pH 6.0 or EDTA pH 8.0) may improve staining. Validate in the relevant tissue and sample system.
• Cross‑reacts with mouse and rat per supplier specifications; verify reactivity and optimal dilution for each species experimentally.
• As a polyclonal antibody, some lot‑to‑lot variability may occur; always titrate and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing channel for diagnostic developers and research labs, offering this anti‑TNFRSF11A antibody alongside a comprehensive portfolio of IVD raw materials. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance in sourcing rare‑target raw materials for osteoimmunology and NF‑κB signaling projects, CamelBio supports your workflow from concept to clinic. Contact us to discuss customized supply solutions and technical consultation tailored to your assay development needs.