Anti-TNFRSF10D Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UBN6

Core Product Specifications and Parameters

Biological Background: TNFRSF10D Function and Localization

• TNFRSF10D, also known as decoy receptor 2 (DcR2), TRAIL receptor 4 (TRAIL-R4), CD264, or TRUNDD, is a receptor for the cytotoxic ligand TRAIL. Related references: PMID:9430226

• It contains a truncated death domain and thus cannot transduce apoptotic signals; instead, it acts as a decoy receptor that protects cells against TRAIL-induced apoptosis. Related references: PMID:9537512

• Studies are contradictory regarding its ability to activate NF-kappa-B: some indicate no activation, while others suggest it can induce the pathway. Related references: PMID:9382840, PMID:9430226

• It is a single-pass type I membrane protein.

• The protein is widely expressed across tissues, including fetal kidney, lung, liver, adult testis, liver, peripheral blood leukocytes, colon, small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta, and heart.

• As a member of the TNF receptor superfamily, it possesses extracellular cysteine-rich repeats, is glycosylated, and contains disulfide bonds. It also features a signal peptide and a transmembrane helix.

• The theoretical molecular weight is approximately 42 kDa (41849 Da), consistent with the observed band.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular domain (amino acids 257-386), so the antibody may recognize the full-length receptor. For Western blot, consider testing a dilution range and validate with appropriate controls.
• For IHC-P, antigen retrieval may be required; paraffin-embedded human or mouse tissues can be utilized.
• In ELISA, plate coating with recombinant protein or cell lysates may be employed; optimize coating conditions.
• Cross-reactivity with mouse is reported; the antibody may be suitable for mouse models, but validate in the relevant sample system.
• Store at -20°C as recommended; avoid repeated freeze-thaw cycles to preserve activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined path to IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. This TNFRSF10D antibody supports apoptosis research and assay development, fitting into our portfolio of cancer- and immune-related IVD reagents. For any custom sourcing or technical consultation, our team is ready to assist.