Anti-TNFRSF10C Rabbit Polyclonal Antibody for WB, ELISA - O14798

Core Product Specifications and Parameters

Biological Background: TNFRSF10C Function and Localization

• TNFRSF10C (Decoy Receptor 1, DcR1; also called TRAIL-R3, CD263, TRID) is a member of the tumor necrosis factor receptor superfamily that functions as a decoy receptor for the cytotoxic ligand TRAIL (TNF-related apoptosis-inducing ligand).
• Unlike the functional death receptors TRAIL-R1 (TNFRSF10A) and TRAIL-R2 (TNFRSF10B), TNFRSF10C lacks an intracellular death domain and therefore cannot trigger apoptosis upon TRAIL binding.
• By competitively binding TRAIL, TNFRSF10C protects cells from TRAIL-mediated apoptosis, acting as a negative regulator of extrinsic cell death signaling.
• The protein is anchored to the plasma membrane via a C-terminal glycosylphosphatidylinositol (GPI) linkage rather than a transmembrane domain, and according to UniProt, its subcellular location is the cell membrane.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, consistent with its extracellular function and structural stability.
• TNFRSF10C is abundantly expressed in normal human tissues, particularly in peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung, and heart, often at higher levels than in tumor cell lines.
• The receptor may contribute to immune privilege and tumor evasion by reducing TRAIL sensitivity in certain tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 150–238 of human TNFRSF10C, which lies within the extracellular domain; this region is expected to be accessible in native membrane-bound or soluble forms.
• For Western blot, the predicted molecular weight is approximately 27 kDa, but glycosylation may lead to a higher apparent mass; include proper positive controls (e.g., human peripheral blood leukocyte lysates) and validate the banding pattern.
• This antibody is suitable for ELISA applications; pair it with a matched detection antibody for optimal sensitivity when quantifying soluble or membrane-derived TNFRSF10C.
• Cross-reactivity with mouse and rat is documented; consider sequence alignment to confirm epitope conservation and validate in the relevant rodent model system before quantitative use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of IVD raw materials for diagnostic manufacturers and research laboratories. As your integrated sourcing partner, we offer bulk quantities of validated antibodies for apoptosis and cancer-related targets such as TNFRSF10C, along with customized monoclonal/polyclonal antibody development, matched antibody pairs, and ancillary reagents. Whether you need routine supply or assistance with rare target sourcing, CamelBio provides a single-channel solution from concept to commercialization.