Anti-TNFR2/TNFRSF1B Rabbit Polyclonal Antibody for WB, ELISA - P20333

Core Product Specifications and Parameters

Biological Background: TNFR2/TNFRSF1B Function and Localization

• Tumor necrosis factor receptor superfamily member 1B (TNFR2/TNFRSF1B), also known as p75, CD120b, or TNF-R-II, is encoded by the TNFRSF1B gene in humans.
• TNFR2 functions as a high-affinity receptor for tumor necrosis factor (TNF/TNFSF2) and has approximately 5-fold lower affinity for lymphotoxin-alpha (TNFSF1).
• The receptor mediates most of the metabolic effects of TNF, and its cytoplasmic domain recruits the TRAF1/TRAF2 complex, which in turn recruits the apoptotic suppressors BIRC2 and BIRC3 to modulate apoptotic signaling.
• An alternatively spliced isoform (isoform 2) acts as a dominant-negative regulator by blocking TNF-induced apoptosis, suggesting a role in fine-tuning TNF responses.
• TNFR2 is primarily localized at the cell membrane but can also be found in secreted forms, consistent with its function as a soluble decoy receptor.
• The protein undergoes several post-translational modifications, including phosphorylation and glycosylation, and contains disulfide bonds characteristic of the TNFR superfamily.

Experimental Guidance and Technical Tips

• The immunogen spans the cytoplasmic region of human TNFR2 (aa 295-377), which is conserved across species; this region may contribute to the antibody’s observed cross-reactivity with mouse and rat orthologs.
• For Western blot (WB) analysis, consider using whole-cell lysates or tissue extracts expressing TNFR2; endogenous expression may be low in some cell types, so induction or enrichment might be necessary.
• In ELISA applications, the antibody can be utilized in either direct or capture formats; however, for sandwich ELISA, a compatible antibody pair recognizing distinct epitopes should be validated.
• Given the presence of a secreted isoform, experimental design should account for both membrane-bound and soluble receptor forms when interpreting results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your tumor necrosis factor receptor research and diagnostic development by offering this high-quality TNFR2/TNFRSF1B antibody along with a comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. As a one-stop IVD raw-material sourcing partner, we simplify procurement from concept to clinic, including rare target raw-material sourcing to accelerate your assay development.