Anti-TNFR2/TNFRSF1B Rabbit Monoclonal Antibody for WB, ELISA - P20333

Core Product Specifications and Parameters

Biological Background: TNFR2/TNFRSF1B Function and Localization

• Tumor necrosis factor receptor superfamily member 1B (TNFR2/TNFRSF1B) is a single-pass type I membrane protein, also known as p75, TNF-R-II, and CD120b.
• It functions as a high-affinity receptor for tumor necrosis factor alpha (TNF-α) and a lower-affinity receptor for homotrimeric lymphotoxin-alpha (TNFSF1).
• Upon ligand binding, the TRAF1/TRAF2 complex is recruited, which in turn recruits the apoptotic suppressors BIRC2 and BIRC3, mediating most of the metabolic effects of TNF.
• An alternative splicing isoform (Isoform 2) acts as a soluble decoy receptor that blocks TNF-induced apoptosis, suggesting a role in regulating TNF biological activity through antagonism.
• Localizes predominantly to the cell membrane, but secreted forms have also been detected.
• Known post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation.
• Classified under keywords including apoptosis, alternative splicing, glycoprotein, pharmaceutical, and receptor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 362–461 of human TNFR2, a region likely in the cytoplasmic domain. This design supports detection of both membrane-bound and intracellular forms in Western blot and ELISA.
• For Western blot, expect a band around 48 kDa under reducing conditions; validate in relevant human cell lines or tissue lysates positive for TNFR2.
• For sandwich ELISA applications, consider pairing with a validated capture antibody targeting a distinct epitope; confirm linearity and specificity using recombinant human TNFR2 or biological fluids.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio supports diagnostic manufacturers and researchers from concept to clinic. Beyond this TNFR2/TNFRSF1B antibody, we offer validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents for TNF signaling and apoptosis research. Our supply capabilities extend to rare-target raw materials, ensuring streamlined procurement for your immunological assay development.