Anti-TNFAIP3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P21580

Core Product Specifications and Parameters

Biological Background: TNFAIP3 Function and Localization

• Tumor necrosis factor alpha-induced protein 3 (TNFAIP3), also known as A20, is a ubiquitin-editing enzyme that possesses both ubiquitin ligase and deubiquitinase activities.
• It plays a critical role in terminating NF-kappa-B activation in immune and inflammatory responses triggered by cytokines (e.g., TNF, IL-1 beta) and pathogen-associated molecular patterns via Toll-like receptors (TLRs).
• TNFAIP3 functions within a ubiquitin-editing complex including RNF11, ITCH, and TAX1BP1 to ensure transient inflammatory signaling. It deubiquitinates 'Lys-63'-linked polyubiquitin chains on signaling intermediates such as RIPK1, TRAF6, and MALT1, while catalyzing 'Lys-48'-linked polyubiquitin chains to target proteins for proteasomal degradation.
• It also exhibits non-catalytic inhibition of the I-kappa-B-kinase (IKK) complex through polyubiquitin-mediated association with NEMO (IKBKG), preventing MAP3K7-mediated phosphorylation.
• Subcellularly, TNFAIP3 is found in the cytoplasm, nucleus, and lysosome, reflecting its multifunctional roles.
• Key post-translational modifications include phosphorylation and acetylation; it contains zinc-finger domains and a deubiquitinase OTU domain, classifying it as a zinc-binding thiol protease and transferase.
• Mutations in TNFAIP3 are associated with autoimmune and inflammatory diseases, and it acts as an inhibitor of programmed cell death.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 600-738 of human TNFAIP3, a region likely containing both zinc-finger and OTU domain motifs; thus, the antibody may recognize both full-length and possibly truncated isoforms.
• For Western blot (WB), the predicted molecular weight is approximately 90 kDa; validate with appropriate positive and negative controls (e.g., stimulated vs. unstimulated cells) given the inducible nature of A20.
• In immunohistochemistry (IHC-P), consider antigen retrieval methods and optimize dilution; note A20 cytoplasmic and nuclear localization.
• For ELISA, pairing with a capture antibody may be feasible; however, as a polyclonal, lot-to-lot consistency should be monitored.
• Cross-reactivity with rat is specified; confirm reactivity in your specific sample system via pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-TNFAIP3 antibody supports research on inflammatory signaling, autoimmune disease mechanisms, and apoptosis regulation. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized rare-target raw-material sourcing to accelerate your assay development and production.