Anti-TNFAIP1 Polyclonal Antibody for WB and ELISA - Q13829

Core Product Specifications and Parameters

Biological Background: TNFAIP1 Function and Localization

• TNFAIP1 (also known as BTB/POZ domain-containing protein TNFAIP1, Protein B12, or hBACURD2) is encoded by the TNFAIP1 gene (GeneID 7126) and exists as multiple isoforms via alternative splicing.
• It functions as a substrate-specific adapter in the BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex, mediating the ubiquitination of RHOA, which targets it for proteasomal degradation and thereby regulates the actin cytoskeleton and cell migration.
• Interaction of TNFAIP1 with RHOB may play a role in regulating apoptosis.
• Additionally, TNFAIP1 may enhance PCNA-dependent DNA polymerase delta activity.
• Subcellularly, TNFAIP1 localizes to the cytoplasm, nucleus, and endosomes.
• The protein is subject to phosphorylation and participates in the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen (aa 90–160) spans a region within the N-terminal BTB/POZ domain and adjacent sequences, which may aid in recognizing human TNFAIP1 isoforms and variants; cross-reactivity with other species is not guaranteed.
• For Western blot, a band of approximately 36 kDa is expected; optimize sample preparation and primary antibody dilution for your specific sample type.
• In ELISA applications, perform titration experiments to determine optimal coating antigen concentration and antibody working dilution.
• As a polyclonal antibody, it may offer enhanced sensitivity for detecting low-abundance targets but should be validated for specificity in the intended assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined supply chain for diagnostic and research raw materials, including this rabbit anti-TNFAIP1 polyclonal antibody, to support investigations into Rho GTPase signaling and ubiquitin-mediated degradation. Beyond antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal reagents, ancillary bulk materials, and specialized sourcing for challenging targets—bringing together every component needed to advance your assay from concept to clinic.