Anti-TNFα Rabbit Polyclonal Antibody for WB and ELISA - P16599

Core Product Specifications and Parameters

Biological Background: TNFα Function and Localization

• TNFα, also known as cachectin or tumor necrosis factor ligand superfamily member 2, is a multifunctional cytokine primarily secreted by activated macrophages.
• It mediates its biological effects by binding to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR, triggering pathways that can lead to apoptosis or cell survival.
• This cytokine is a potent pyrogen, causing fever directly and by stimulating interleukin-1 secretion, and is implicated in cachexia induction.
• TNFα plays a role in insulin resistance by inhibiting insulin-induced IRS1 tyrosine phosphorylation and promoting GKAP42 protein degradation in adipocytes.
• It synergistically promotes angiogenesis with IL1B and IL6 by inducing VEGF production, and also stimulates osteoclastogenesis, thereby mediating bone resorption.
• The intracellular domain (ICD) form of TNFα, generated by proteolytic processing, can induce IL12 production in dendritic cells.
• Subcellularly, TNFα is localized at the cell membrane as a transmembrane protein, but can be secreted as a soluble form after cleavage.
• Key post-translational modifications include glycosylation, myristoylation, phosphorylation, and disulfide bond formation, which are critical for its structure and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 80-235 of rat TNFα, likely covering the extracellular domain; consider that the polyclonal antibody may detect both the precursor and mature secreted forms.
• For Western blot, a band around 26kDa is expected; validate the antibody in the relevant sample system to confirm specificity, as additional bands from processing intermediates or cross-reactivity may appear.
• Given the predicted wide cross reactivity, verify detection of TNFα from human, mouse, and other species using appropriate positive controls.
• Optimization of dilution factors and incubation conditions is recommended for each assay (WB, ELISA) and biological matrix.

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CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive sourcing solution for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-TNFα product, and bulk ancillary reagents. Our expertise in inflammation and immunology targets helps accelerate your assay development from concept to clinic. Contact CamelBio for reliable access to rare target antibodies and customized supply services.