Anti-TNF RII/TNFRSF1B/CD120b Polyclonal Antibody for WB, ELISA - P25119

Core Product Specifications and Parameters

Biological Background: TNF RII/TNFRSF1B/CD120b Function and Localization

• TNFRSF1B is a member of the tumor necrosis factor receptor superfamily, also known as TNF-R2, p75, or CD120b, and functions as a high-affinity receptor for TNFSF2/TNF.
• The receptor engages homotrimeric TNFSF1/lymphotoxin-alpha with approximately 5-fold lower affinity, contributing to distinct signaling outcomes.
• Upon ligand binding, it recruits the TRAF1/TRAF2 adaptor complex, which subsequently associates with the apoptotic suppressors BIRC2 and BIRC3 to regulate cell survival.
• TNFRSF1B is a transmembrane protein localized to the cell membrane, consistent with its role in extracellular signal transduction.
• Key post-translational modifications include phosphorylation and glycosylation, which may modulate receptor stability, trafficking, or ligand interaction.
• The protein contains characteristic structural features such as disulfide bonds, a signal peptide, and transmembrane helix, typical of the TNFR superfamily.
• As a receptor involved in inflammatory and immune responses, TNFRSF1B is implicated in pathways controlling apoptosis, NF-κB activation, and cell proliferation.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 23-258), targeting a region likely accessible for detection in native and denaturing assays; consider this when designing experiments.
• For Western blotting, use reducing conditions and anticipate a band near the predicted molecular weight of ~50 kDa; however, glycosylation may cause the observed band to shift higher.
• Validate antibody specificity using cell lines or tissues known to express TNFRSF1B, such as immune cells or relevant mouse/human samples.
• In ELISA applications, verify the antibody pair compatibility and consider using recombinant TNFRSF1B protein as a positive control.
• Since the antibody cross-reacts with human and mouse, it is suitable for comparative studies but may require species-specific optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes as a one-stop partner for IVD raw-material sourcing, from discovery to clinical application. For researchers exploring TNF signaling and apoptosis pathways, we provide this anti-TNFRSF1B antibody alongside a broad portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing. Our supply capabilities are designed to streamline your procurement and accelerate your project timelines.