Anti-TNF-α Monoclonal Antibody for IHC-P, ELISA - P01375

Core Product Specifications and Parameters

Biological Background: TNF-α Function and Localization

• TNF-α, also known as cachectin or TNFSF2, is a member of the tumor necrosis factor (TNF) superfamily, encoded by the TNF gene in humans.
• As a key cytokine mainly secreted by macrophages, it binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR to mediate diverse biological effects, including cell death, inflammation, and immune regulation.
• It acts as a potent pyrogen, inducing fever, and is implicated in cachexia; under certain conditions, it can also stimulate cell proliferation and differentiation.
• TNF-α impairs regulatory T-cell (Treg) function in rheumatoid arthritis by up-regulating protein phosphatase 1 (PP1), which dephosphorylates FOXP3 at Ser-418, rendering Tregs defective (PubMed:23396208). Related references: PMID:23396208
• It promotes angiogenesis by synergistically inducing VEGF production with IL1B and IL6 (PubMed:12794819), and it drives osteoclastogenesis, linking it to bone resorption. Related references: PMID:12794819
• The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells, highlighting a non-canonical signaling role.
• Subcellular localization includes the cell membrane, membrane, and secretion; it exists as a transmembrane precursor that is cleaved to release the soluble cytokine.
• Post-translational modifications, such as glycosylation, myristoylation, phosphorylation, and disulfide bond formation, are critical for its stability and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 77-233, representing the extracellular domain of the mature, secreted form of human TNF-α. This design ensures reactivity with the soluble cytokine.
• For IHC-P, consider antigen retrieval protocols optimized for human tissue samples, and validate expression in positive controls such as inflamed lymph nodes or synovial tissue.
• In ELISA, this antibody may serve as a capture or detection reagent; establish optimal conditions by pairing with a suitable matched antibody and testing recombinant TNF-α standards.
• Given the targeted region, confirm reactivity with the native trimeric form of TNF-α in your assay system, as the immunogen covers the receptor-binding domain.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, technical services, and consulting. As a trusted sourcing partner, we offer validated monoclonal antibodies like this anti-TNF-α clone, along with optimized antibody pairs, bulk ancillary reagents, and custom development for inflammation and cytokine-related biomarkers. Whether you need a specific clone for assay development or rare target raw materials, CamelBio delivers one-stop solutions from concept to clinic.