Anti-TMX1 Rabbit Polyclonal Antibody for WB, ELISA - Q9H3N1

Core Product Specifications and Parameters

Biological Background: TMX1 Function and Localization

• Thioredoxin-related transmembrane protein 1 (TMX1), also known as TXNDC1 or PDIA11, is a member of the thioredoxin superfamily and functions as a protein disulfide isomerase. Related references: PMID:11152479, PMID:37648867

• It catalyzes dithiol-disulfide exchange reactions via its redox-active center, playing a critical role in the formation and reduction of disulfide bonds, particularly for transmembrane proteins at the endoplasmic reticulum membrane. Related references: PMID:22228764

• TMX1 acts as a key inhibitor of the alternative triglyceride biosynthesis pathway by suppressing TMEM68/DIESL activity at the endoplasmic reticulum, thereby limiting triacylglycerol accumulation. Related references: PMID:37648867

• It is involved in endoplasmic reticulum-associated degradation (ERAD) by facilitating the processing of folding-defective polypeptides through its isomerase activity. Related references: PMID:29932915

• Upon secretion, TMX1 negatively regulates platelet aggregation in the extracellular space. Related references: PMID:30425049

• TMX1 localizes to the endoplasmic reticulum membrane, mitochondrial membrane, and can be secreted. It acts as a regulator of ER-mitochondria contact sites and controls Ca2+ flux between these organelles. Related references: PMID:27502484, PMID:31304984

• Ubiquitously expressed with highest levels in kidney, liver, placenta, and lung. Related references: PMID:11152479

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 210–280 of human TMX1; consider epitope accessibility in native versus denaturing conditions for WB.
• For WB, TMX1 has a predicted molecular weight of ~32 kDa; confirm band size in relevant human cell or tissue lysates.
• Optimize antibody dilution and detection conditions for your specific sample type, as TMX1 may exist in membrane, secreted, or mitochondrial fractions.
• Validate cross-reactivity in your assay system if non-human samples are to be tested (cross-reactivity provided only for human).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibodies such as this TMX1 rabbit polyclonal antibody for studies on protein disulfide isomerases, ER biology, and metabolism. Our comprehensive supply chain includes bulk antibodies, optimized antibody pairs, and ancillary reagents to support your assay development from research to clinical use.