Anti-TMX1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9H3N1

Core Product Specifications and Parameters

Biological Background: TMX1 Function and Localization

• TMX1, also known as thioredoxin domain-containing protein 1 (TXNDC1) or protein disulfide-isomerase TMX1, is a member of the thioredoxin superfamily.
• TMX1 catalyzes dithiol-disulfide exchange reactions through its active center dithiol (PubMed:11152479, PubMed:37648867). Related references: PMID:11152479, PMID:37648867
• Acts as a key inhibitor of the alternative triglyceride biosynthesis pathway by inhibiting TMEM68/DIESL at the endoplasmic reticulum, thereby restricting triacylglycerol accumulation (PubMed:37648867). Related references: PMID:37648867
• Possesses protein disulfide isomerase activity, mediating the formation or reduction of disulfide bonds at the endoplasmic reticulum membrane, and is involved in ER-associated degradation (ERAD) of folding-defective polypeptides (PubMed:22228764, PubMed:29932915). Related references: PMID:22228764, PMID:29932915
• Secreted TMX1 acts as a negative regulator of platelet aggregation (PubMed:30425049). Related references: PMID:30425049
• Regulates ER-mitochondria contact sites and Ca2+ flux via redox signaling (PubMed:27502484, PubMed:31304984). Related references: PMID:27502484, PMID:31304984
• Subcellularly localizes to the endoplasmic reticulum membrane, mitochondrial membrane, and is secreted. Ubiquitously expressed with high levels in kidney, liver, placenta, and lung (PubMed:11152479). Related references: PMID:11152479
• Keywords: Disulfide bond, electron transport, endoplasmic reticulum, isomerase, lipoprotein, membrane, mitochondrion, palmitate, phosphoprotein, redox-active center, transmembrane helix.

Experimental Guidance and Technical Tips

• Consider using this antibody for Western blot detection of TMX1 in human, mouse, and rat samples, given its cross-reactivity profile.
• Optimize dilution factors for WB and IHC-P empirically; the immunogen spans a region conserved across these species, which may contribute to cross-reactivity.
• For ELISA, ensure the antigen coating matches the immunogen region to avoid epitope masking.
• Validate in relevant tissue sample systems, such as kidney or liver tissues where TMX1 is highly expressed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for redox biology, ER stress, and metabolic disease research. With expertise in rare target sourcing and seamless procurement, we support diagnostic manufacturers in advancing their assay development from concept to clinic. Explore our TMX1-related offerings to streamline your next project.