Anti-TMTC3 Polyclonal Antibody for WB and ELISA - Q6ZXV5

Core Product Specifications and Parameters

Biological Background: TMTC3 Function and Localization

• TMTC3 is a protein O-mannosyl-transferase that catalyzes the transfer of mannose to serine or threonine residues of target proteins, including cadherins and PDIA3 (PubMed:28973932). Related references: PMID:28973932
• It is a member of the TMTC family of four O-mannosyltransferases, each playing a distinct role in decorating cadherin domains with O-linked mannose glycans.
• TMTC3 is involved in the positive regulation of proteasomal protein degradation in the endoplasmic reticulum (ER) and helps control ER stress responses.
• The protein localizes to the endoplasmic reticulum membrane and contains several tetratricopeptide repeat (TPR) domains and transmembrane helices.
• TMTC3 undergoes alternative splicing and is subject to phosphorylation and glycosylation.
• Genetic variants in TMTC3 are associated with lissencephaly, a neurological disorder, highlighting its importance in neural development.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, consider using standard reducing SDS-PAGE conditions; the predicted molecular weight of TMTC3 is approximately 104 kDa. Validate the antibody in your specific sample system, as the immunogen corresponds to amino acids 615-914.
• For ELISA applications, the antibody can be tested as a capture or detection reagent; optimal coating concentrations and dilutions should be determined experimentally.
• Cross-reactivity has been confirmed for human TMTC3; reactivity with other species or isoforms has not been tested and should be verified if needed.
• As a polyclonal antibody, lot-to-lot consistency should be confirmed for critical quantitative assays; consider using a monoclonal antibody pair if high reproducibility is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and laboratories, offering one-stop access to high-quality raw materials like this TMTC3 polyclonal antibody for studying O-mannosylation and ER stress. Beyond individual antibodies, we provide validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and custom sourcing for rare targets—supporting your assay development from concept to clinic.