Anti-TMPRSS6 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q8IU80

Core Product Specifications and Parameters

Biological Background: TMPRSS6 Function and Localization

• TMPRSS6, also known as matriptase-2, is a transmembrane serine protease encoded by the TMPRSS6 gene. Related references: PMID:18976966 PMID:20518742 PMID:25156943
• It cleaves cell surface hemojuvelin (HJV), a key regulator of hepcidin expression, thereby controlling systemic iron homeostasis. Related references: PMID:18408718 PMID:18976966 PMID:25156943
• The protein is anchored at the cell membrane via a signal-anchor and a transmembrane helix.
• TMPRSS6 is a multidomain serine protease containing disulfide bonds, glycosylation sites, and a serine protease domain; it is synthesized as a zymogen and activated by proteolytic cleavage.
• Alternative splicing produces multiple isoforms, and mutations in the TMPRSS6 gene cause iron-refractory iron deficiency anemia (IRIDA).
• With a calculated molecular weight of approximately 90 kDa, the protein includes characteristic repeats and is classified under reference proteome.

Experimental Guidance and Technical Tips

• For Western blot, consider a standard dilution range for rabbit polyclonal antibodies and validate with relevant positive cell or tissue lysates expressing TMPRSS6.
• Due to the transmembrane nature of the target, include a membrane protein extraction protocol if the signal is weak in whole-cell lysates.
• In immunofluorescence (IF-P), use appropriate fixation/permeabilization conditions suitable for membrane proteins; confirm specificity with knockout or knockdown controls.
• The immunogen (amino acids 77–220) may detect multiple isoforms or proteolytic fragments; verify band patterns on blots against expected molecular weights.
• Given the listed cross-reactivity, this antibody is suitable for mouse and rat samples; however, always confirm reactivity in your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a curated portfolio of IVD raw materials, including validated polyclonal antibodies like this TMPRSS6 antibody, to support diagnostic manufacturers and research laboratories. Our offerings span bulk ancillary reagents, optimized antibody pairs, and rare target sourcing, all backed by technical consulting from concept to clinical development. Whether your work involves iron metabolism assays or broader protease research, CamelBio simplifies procurement with a single, reliable source.