Anti-TMPRSS2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O15393

Core Product Specifications and Parameters

Biological Background: TMPRSS2 Function and Localization

• TMPRSS2 is a plasma membrane-anchored serine protease that cleaves at arginine residues, playing a key role in proteolytic cascades. (PubMed:32703818, PubMed:35676539, PubMed:37990007) Related references: PMID:32703818 PMID:35676539 PMID:37990007
• Androgen-induced TMPRSS2 activates several substrates, including pro-hepatocyte growth factor/HGF, protease activated receptor-2/F2RL1, and matriptase/ST14, leading to extracellular matrix disruption and metastasis of prostate cancer cells. (PubMed:15537383, PubMed:25122198, PubMed:26018085) Related references: PMID:15537383 PMID:25122198 PMID:26018085
• TMPRSS2 facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections by proteolytic cleavage of ACE2 receptor and coronavirus spike glycoproteins, which promotes viral uptake and entry. (PubMed:24227843, PubMed:32142651, PubMed:32404436) Related references: PMID:24227843 PMID:32142651 PMID:32404436
• The protease also cleaves and activates spike glycoproteins of other human coronaviruses (HCoV-229E, HCoV-EMC) and fusion glycoproteins of Sendai virus, human metapneumovirus, and human parainfluenza viruses, and is essential for influenza A virus hemagglutinin activation.
• TMPRSS2 acts as a receptor for human coronavirus HKU1-CoV, synergizing with disialoside glycans to facilitate viral entry; the viral S protein binds to the inactive form of TMPRSS2 and inhibits its protease activity.
• Subcellularly, TMPRSS2 is a type II transmembrane protein located at the cell membrane and can be secreted.
• TMPRSS2 is highly expressed in prostate, lung type II pneumocytes, and small intestine, with coexpression with ACE2 in tissues relevant to SARS-CoV-2 infection, such as lung, ileal absorptive enterocytes, and nasal goblet secretory cells.

Experimental Guidance and Technical Tips

• The immunogen used to raise this antibody corresponds to amino acids 1-100 of human TMPRSS2. This region may be conserved across human, mouse, and rat (as indicated by cross reactivity), supporting its use in multiple species.
• For Western blot (WB), the predicted molecular weight is approximately 54 kDa; however, glycosylation and potential autoproteolytic processing may result in additional bands—optimize loading and detection conditions.
• For immunohistochemistry (IHC-P), consider appropriate antigen retrieval methods and validate signal specificity using positive (e.g., prostate or lung tissue) and negative controls.
• This product is also suitable for ELISA; users should empirically determine optimal coating and detection conditions for their specific setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers and research institutes with a comprehensive range of IVD raw materials and technical support. This TMPRSS2 monoclonal antibody supports investigations in host-virus interactions, cancer biology, and protease research. Beyond individual reagents, CamelBio sources validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assists with rare target raw-material procurement to streamline your development pipeline.