Anti-TMPRSS2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15393

Core Product Specifications and Parameters

Biological Background: TMPRSS2 Function and Localization

• Transmembrane protease serine 2 (TMPRSS2) is a plasma membrane-anchored serine protease that cleaves at arginine residues. Related references: PMID:32703818, PMID:35676539, PMID:37990007
• Participates in proteolytic cascades of relevance for the normal physiologic function of the prostate. Related references: PMID:25122198
• Androgen-induced TMPRSS2 activates several substrates including pro-hepatocyte growth factor/HGF, protease activated receptor-2/F2RL1, and matriptase/ST14, leading to extracellular matrix disruption and metastasis of prostate cancer cells. Related references: PMID:15537383, PMID:25122198, PMID:26018085
• Facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via proteolytic cleavage of ACE2 receptor and spike glycoproteins, promoting viral uptake and syncytia formation. Related references: PMID:24227843, PMID:32142651, PMID:32404436, PMID:32703818, PMID:33051876, PMID:34159616, PMID:35676539
• Cleaves and activates spike glycoproteins of other human coronaviruses (HCoV-229E, HCoV-EMC) and fusion glycoproteins of Sendai virus, human metapneumovirus, and human parainfluenza viruses; essential for influenza A virus infectivity via hemagglutinin cleavage.
• Acts as a receptor for human coronavirus HKU1-CoV, synergizing with disialoside glycans; viral S protein binds inactive TMPRSS2 and inhibits its protease activity.
• Subcellular localization includes the cell membrane and secreted forms.
• Expressed in several epithelial tissues including prostate, lung type II pneumocytes, small intestine, colon, stomach, and salivary gland; coexpressed with ACE2 in lung, ileal, intestinal, corneal, gallbladder, and nasal secretory cells.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100); the antibody may detect both full-length and cleaved forms. Validate cleavage products in your experimental system.
• For Western blot, the predicted molecular weight is approximately 54 kDa; glycosylation and other modifications may cause mobility shifts. Use appropriate positive controls (e.g., prostate tissue, LNCaP cells).
• For IHC-P and IF/ICC, titrate the antibody and include known expressing tissues (prostate, lung) as positive controls.
• For ELISA, consider using recombinant protein or peptide as coating antigen and include negative controls to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a reliable supply of validated TMPRSS2 antibodies and ancillary reagents. Our portfolio includes polyclonal and monoclonal options optimized for applications such as WB, IHC, and ELISA. For customized immunodetection solutions or bulk orders, contact our team for technical consultation and procurement support.