Anti-TMPO Rabbit Polyclonal Antibody - P42166 / P42167

Core Product Specifications and Parameters

Biological Background: TMPO (Lamina-associated polypeptide 2) Function and Localization

• TMPO encodes thymopoietin, a protein that exists as multiple isoforms (α, β, γ) due to alternative splicing, with molecular weights of approximately 75 kDa (isoform α), 51 kDa (isoform β), and 39 kDa (isoform γ). The immunogen for this antibody corresponds to the α isoform.
• Isoform α, known as Lamina-associated polypeptide 2α (LAP2α), is a key nuclear protein involved in the structural organization of the nucleus and post-mitotic nuclear assembly. It plays a critical role, together with LMNA, in anchoring the retinoblastoma protein (RB1) within the nucleus.
• Thymopoietin-derived peptides TP and TP5 exhibit immunomodulatory functions, potentially influencing T-cell development and function. This suggests a regulatory role beyond nuclear architecture.
• TMPO is predominantly localized in the nucleus and associates with chromosomes, consistent with its roles in nuclear envelope dynamics and chromatin organization.
• The protein is expressed in many tissues, with the highest abundance in adult thymus and fetal liver, reflecting its dual functions in nuclear structure and immune system development.
• Post-translational modifications of TMPO include acetylation, methylation, and phosphorylation, which may regulate its stability, interactions, and functional activity.
• Mutations in the TMPO gene have been linked to dilated cardiomyopathy (CMD1T), highlighting its importance beyond nuclear organization to cardiac muscle physiology.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering amino acids 1–410 of human TMPO (NP_001027454.1), representing the N-terminal region of isoform α. This region may recognize shared epitopes across isoforms, allowing detection of multiple TMPO variants.
• In Western blot analysis, anticipate bands corresponding to the classic TMPO isoforms: ~75 kDa (isoform α), ~50 kDa (isoform β), and ~38 kDa (isoform γ). Actual band patterns will depend on the cell line or tissue due to differential isoform expression.
• For immunohistochemistry (IHC) and immunofluorescence (IF), nuclear staining is expected. Validation in tissues with high TMPO expression, such as thymus, liver, or heart, is recommended.
• This antibody has shown cross-reactivity with mouse and rat, but sequence homology in the target epitope region should be verified for your specific model system.
• As a polyclonal antibody, batch-to-batch consistency should be monitored. Consider titration experiments to optimize conditions for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing platform for IVD raw materials, connecting diagnostic manufacturers and research institutes with high-quality reagents and technical support. This anti-TMPO antibody supports studies in nuclear organization, laminopathies, and immunology, and is part of an extensive portfolio of validated antibodies for disease-relevant targets. From bulk quantities of polyclonal antibodies to ancillary reagents and custom rare-target sourcing, CamelBio streamlines procurement to accelerate your assay development from concept to clinic.