Anti-TMF1 Rabbit Polyclonal Antibody for WB, ELISA - P82094

Core Product Specifications and Parameters

Biological Background: TMF1 Function and Localization

• TMF1 (TATA element modulatory factor), also designated ARA160, is a potential coactivator of the androgen receptor, linking it to hormone-responsive transcription.
• It mediates the degradation of STAT3, thereby modulating JAK/STAT signaling cascades with implications for immune response and oncogenesis.
• The protein plays critical roles in two distinct RAB6-dependent retrograde transport pathways: from endosomes to the Golgi and from the Golgi to the endoplasmic reticulum, underscoring its importance in intracellular trafficking.
• TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation driven by the TATA-binding protein (TBP), functioning as a transcriptional repressor in viral and cellular contexts.
• It localizes to the cytoplasm, nucleus, and Golgi apparatus membrane, consistent with its dual involvement in gene regulation and membrane dynamics.
• Structural features include coiled-coil domains and multiple phosphorylation sites; it is classified as a DNA-binding phosphoprotein subject to alternative splicing, enhancing functional diversity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 900–1000); consider that the antibody may recognize endogenous TMF1 across isoforms with this conserved region.
• For Western blotting, whole-cell lysates from human or mouse cell lines (e.g., HeLa, Jurkat, NIH/3T3) are suitable, given the predicted cross-reactivity; validate in your specific sample system.
• The predicted molecular weight is approximately 123 kDa, but post-translational modifications (e.g., phosphorylation) may cause a shift; use appropriate molecular weight markers.
• For ELISA, the peptide immunogen can serve as a positive control; titrate the antibody using standard indirect ELISA protocols to determine an optimal working concentration.
• This polyclonal antibody is recommended for WB and ELISA; performance in other applications (e.g., IF, IP) should be verified empirically by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research laboratories with seamless access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and a broad portfolio of ancillary reagents. For targets like TMF1, involved in transcriptional regulation and protein trafficking, we support your project from concept to clinic by offering rare-target sourcing and bulk-order flexibility. Partner with us to accelerate your assay development with reliable, research-use materials and expert consultation.