Anti-TMEM43 Monoclonal Antibody for WB, IHC-P, ELISA - Q9BTV4

Core Product Specifications and Parameters

Biological Background: TMEM43 Function and Localization

• Encoded by the TMEM43 gene, transmembrane protein 43 (also known as LUMA) is a multi-pass membrane protein of approximately 45 kDa (calculated MW 44876 Da) with diverse roles in nuclear envelope architecture, innate immunity, and cellular signaling.
• Plays a critical role in maintaining nuclear envelope structure by organizing protein complexes at the inner nuclear membrane and is required for retaining emerin (by similarity).
• Modulates innate immune signaling through the cGAS-STING pathway by interacting with RNF26, linking nuclear envelope integrity to antiviral responses. Related references: PMID:32614325
• Functions as a signaling adapter mediating NF-kappa-B activation downstream of EGFR and upstream of CARD10, implicating TMEM43 in inflammatory and proliferative pathways. Related references: PMID:27991920
• Contributes to passive conductance current in cochlear glia-like supporting cells, a gap junction-mediated function essential for normal hearing and speech discrimination. Related references: PMID:34050020
• Predominantly localizes to the endoplasmic reticulum membrane, nucleus inner membrane, and cell membrane, consistent with its roles in nuclear envelope and cellular signaling.
• Exhibits highest tissue expression in placenta, with lower levels detected in heart, ovary, spleen, small intestine, thymus, prostate, and testis.
• Clinically associated with cardiomyopathy, Emery-Dreifuss muscular dystrophy, and non-syndromic deafness; the protein is subject to acetylation and other post-translational modifications (UniProt keywords).

Experimental Guidance and Technical Tips

• For Western blot, expect a specific band at approximately 45 kDa. Positive controls may include human placental tissue lysate or cell lines known to express TMEM43; antibody reactivity has been confirmed for human, mouse, and rat samples.
• In paraffin-embedded immunohistochemistry (IHC-P), antigen retrieval is typically required. Optimal staining may be observed in tissues with high endogenous TMEM43 levels, such as placenta; however, expression in heart, ovary, and other tissues can also be informative after in-house validation.
• For ELISA applications, the recombinant immunogen (aa 35–135) serves as a suitable reference standard. Users should establish assay conditions and linear range through titration experiments.
• Given the monoclonal nature of this antibody, consistent lot-to-lot performance is expected. However, as with any antibody, validation in the specific experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supporting diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials. This anti-TMEM43 monoclonal antibody facilitates investigations into nuclear envelope biology, innate immunity, and auditory function. Beyond this antibody, CamelBio offers a broad portfolio including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials, empowering streamlined development from concept to clinic.