Anti-TMEM25 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q86YD3

Core Product Specifications and Parameters

Biological Background: TMEM25 Function and Localization

• TMEM25 (Transmembrane protein 25) is a single-pass type I transmembrane protein belonging to the immunoglobulin superfamily, with a predicted molecular weight of approximately 39 kDa.
• In neurons, TMEM25 modulates the degradation of the NMDA receptor subunit GRIN2B, thereby playing a critical role in regulating neuronal excitability.
• TMEM25 is localized to multiple cellular compartments, including the cell membrane, late endosomes, and lysosomes, and can also be secreted.
• The protein is post-translationally modified by N-linked glycosylation and contains a signal peptide and disulfide bonds, consistent with its trafficking through the secretory pathway.
• Expression is predominantly in the brain, with high levels in the hippocampal CA1 and CA3 regions, dentate gyrus, cerebellum, and scattered cortical neurons.
• Alternative splicing generates multiple isoforms, which may have distinct functional roles.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 223–322 near the C-terminus; this region is likely accessible for antibody binding in various applications.
• For Western blot (WB), consider using neuronal cell lines or brain tissue lysates, as TMEM25 is highly expressed in the brain. A band around 39 kDa is expected, but glycosylation may cause slight shifts.
• In immunofluorescence/immunocytochemistry (IF/ICC), validate localization by co-staining with markers for the cell membrane, late endosomes, or lysosomes, depending on the experimental context.
• For ELISA, the polyclonal nature may enhance sensitivity, but include appropriate controls to confirm specificity.
• Since the antibody is polyclonal and cross-reacts with mouse and rat, knock-out or knock-down validation is recommended to ensure target specificity in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, providing everything from validated antibody pairs and bulk ancillary reagents to rare target sourcing and technical consulting. Our anti-TMEM25 polyclonal antibody supports neuroscience and neurology research, with potential applications in diagnostic assays targeting NMDA receptor-related pathways. Reach out to our team for tailored sourcing solutions that accelerate your project from concept to clinic.