Anti-TMEM18 Polyclonal Antibody for WB, ELISA - Q96B42

Core Product Specifications and Parameters

Biological Background: TMEM18 Function and Localization

• TMEM18 encodes a transmembrane protein of 16265 Da (UniProt: Q96B42) that functions as a transcription repressor and binds both ssDNA and dsDNA, with preference for GCT and CTG repeats.
• It modulates cell migration, specifically enhancing the glioma-specific migration ability of neural stem cells (NSC) and neural precursor cells (NPC).
• The protein localizes to both the cytoplasm and the nuclear membrane, consistent with its dual roles in transcriptional regulation and signaling.
• Keywords from UniProt indicate it is an alternatively spliced gene, contains coiled coil regions, and has proteomics identification evidence; it is classified as a DNA-binding, transcription, and transmembrane protein.
• No specific post-translational modifications are documented in the UniProt record, but its coiled coil domain likely mediates protein-protein interactions.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide within amino acids 40-100 of human TMEM18. When designing detection or validation assays, consider using recombinant full-length protein or overlapping peptides to confirm specificity.
• This polyclonal antibody is suitable for WB and ELISA. For WB, a band at approximately 16 kDa is expected; pre-validate with appropriate positive and negative controls (e.g., human and rat cell lysates expressing TMEM18).
• Since TMEM18 localizes mainly to the cytoplasm and nuclear membrane, subcellular fractionation may help enrich the signal in WB if endogenous expression is low.
• Cross-reactivity is reported for human and rat; for other species, sequence alignment of the immunogen region is recommended before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw materials, offering a one-stop sourcing bridge from concept to clinic. Beyond this TMEM18 antibody, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your neuroscience, oncology, and transcription research assays. Let our supply capabilities accelerate your diagnostics development with reliable, high-quality materials.