Anti-TMEM176B Rabbit Polyclonal Antibody for WB, ELISA - Q3YBM2

Core Product Specifications and Parameters

Biological Background: TMEM176B Function and Localization

• TMEM176B (Transmembrane protein 176B), also designated LR8 or MS4B2, is a multi-pass membrane protein belonging to the MS4B family.
• It is implicated in the maturation of dendritic cells, potentially influencing antigen presentation and immune regulation.
• The protein is required for the normal development of cerebellar granule cells, as evidenced by similarity-based studies.
• TMEM176B localizes specifically to the nuclear membrane, consistent with its transmembrane topology.
• Tissue expression is observed in lung fibroblasts and dermal fibroblasts, indicating a role in connective tissue or stromal environments.
• The protein undergoes alternative splicing, generating multiple isoforms, and is subject to phosphorylation, suggesting regulated signaling functions.
• It contains several transmembrane helices, anchoring it within lipid bilayers, and has been identified through proteomic analyses.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 148–208 of human TMEM176B; verify that your target isoform encompasses this region.
• For Western blotting, use lysates from human or mouse lung fibroblasts or other TMEM176B-expressing tissues as positive controls.
• Consider pre-incubating the antibody with the recombinant immunogen in a peptide competition assay to confirm band specificity.
• In ELISA applications, titrate the antibody to determine optimal working concentrations for capture or detection formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers and research institutes by providing streamlined access to high-quality IVD raw materials, including validated primary antibodies such as this TMEM176B rabbit pAb. Our portfolio spans optimized monoclonal/polyclonal antibodies, antibody pairs, and bulk ancillary reagents, supporting dendritic cell biology and immuno-oncology research. For bulk orders or customized sourcing of rare targets, CamelBio is your reliable partner from concept to clinic.