Anti-TMEM147 Polyclonal Antibody for WB, ELISA - Q9BVK8

Core Product Specifications and Parameters

Biological Background: TMEM147 Function and Localization

• TMEM147, also known as protein NIFIE14, is a component of the BOS complex and a multi-pass transmembrane protein.
• It serves as a subunit of the multi-pass translocon (MPT) complex, which mediates insertion of multi-pass membrane proteins into the lipid bilayer after the SEC61 complex finishes initial transmembrane segment insertion. Related references: PMID:32820719, PMID:36261522
• Negatively regulates CHRM3 function by interfering with its trafficking to the cell membrane, thereby dampening CHRM3-mediated calcium mobilization and RPS6KA1/p90RSK activation. Related references: PMID:21056967
• Plays a role in regulating LBR localization to the inner nuclear membrane. Related references: PMID:32694168
• Subcellularly, it resides in the endoplasmic reticulum membrane, nucleus membrane, and cell membrane.
• The protein contains multiple transmembrane helices and undergoes alternative splicing.
• Associated with intellectual disability (disease variant keyword), though detailed mechanisms remain under investigation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–100 amino acid region; consider that alternative splicing may generate isoforms lacking this epitope, potentially affecting detection in certain tissues or cell lines.
• For Western blot, a band around 25 kDa is expected; however, endogenous expression levels may vary, so include appropriate positive controls (e.g., human or mouse cell lysates expressing TMEM147).
• Due to its polyclonal nature, lot-to-lot consistency should be monitored; validation in the user's specific sample system is recommended.
• The reported cross-reactivity with mouse enables use in mouse model studies, but epitope conservation across species should be verified if extended to other organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials. This TMEM147 antibody supports investigations into membrane protein biogenesis and related disorders. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target reagents—ensuring a reliable supply chain from concept to clinic.