Anti-TMEM132D Rabbit Polyclonal Antibody for WB, ELISA - Q14C87

Core Product Specifications and Parameters

Biological Background: TMEM132D Function and Localization

• TMEM132D (Transmembrane protein 132D), also known as Mature oligodendrocytes transmembrane protein (MOLT), is encoded by the TMEM132D gene (synonyms: HBE120, KIAA1944) in humans.
• It functions as a regulator of neuronal morphology by inhibiting the WAVE regulatory complex (WCR), a critical complex that controls F-actin cytoskeletal dynamics.
• The protein is a multi-pass membrane protein, predicted to contain several transmembrane helices and a signal peptide, with a molecular weight of approximately 122 kDa.
• Tissue expression studies reveal TMEM132D is expressed in mature oligodendrocytes and detected in brain, lung, pancreas, and testis. High expression is observed in mature neurons of the adult nervous system.
• Post-translational modifications include glycosylation (glycoprotein), and the gene undergoes alternative splicing, producing multiple isoforms.
• Subcellular localization is primarily at the membrane.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody corresponds to amino acids 730-915 of human TMEM132D, a region that shares high homology with mouse and rat orthologs, which may account for the observed cross-reactivity.
• For Western blotting (WB), TMEM132D has a predicted molecular weight of ~122 kDa; ensure adequate gel resolution and transfer conditions for high molecular weight proteins.
• For ELISA applications, titrate the antibody and validate conditions using recombinant TMEM132D or cell lysates from tissues with known expression, such as brain or testis.
• Since TMEM132D is a membrane glycoprotein, consider using mild detergents during lysis and including deglycosylation experiments if band shifts are observed.

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