Anti-TMEM119 Polyclonal Antibody for WB, ELISA - Q4V9L6

Core Product Specifications and Parameters

Biological Background: TMEM119 Function and Localization

• TMEM119 (Transmembrane protein 119), also known as Osteoblast induction factor (OBIF), is a single-pass type I transmembrane protein.
• It plays a critical role in bone formation and mineralization by promoting the differentiation of myoblasts into osteoblasts. Related references: PMID:20025746
• TMEM119 enhances BMP2 production and interacts with the BMP-RUNX2 pathway, leading to upregulation of ATF4, a key transcription factor in osteoblast differentiation.
• Essential for normal spermatogenesis and late testicular differentiation (By similarity).
• Subcellularly, TMEM119 localizes to the cell membrane, cytoplasm, endoplasmic reticulum membrane, and can be secreted.
• Highly expressed in brain microglia and serves as a robust microglia marker; expression is elevated in the brain of patients with Alzheimer's disease.
• Also expressed in osteoblast-like cells in bone tissues and follicular dendritic cells in lymphoid tissues.
• Post-translational modifications include glycosylation and phosphorylation; it contains a signal peptide and a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 160-270, a cytoplasmic region; the resulting antibody likely targets an intracellular epitope. For Western blot, consider using denaturing and reducing conditions; the predicted molecular weight is ~29 kDa, though post-translational modifications (e.g., glycosylation) may cause band shifts.
• For ELISA, direct or indirect coating with recombinant TMEM119 protein or peptide may be appropriate.
• Cross-reactivity with mouse and rat suggests conserved epitope; validate the antibody in rodent cell or tissue lysates.
• Suitable positive controls include osteoblast cell lines (e.g., MC3T3-E1 for mouse), microglia cells, or testis tissue, given TMEM119's biological roles.
• Due to its microglia-specific expression, this antibody could be utilized in neuroscience applications, but confirm specificity in brain samples via additional markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material procurement, offering a comprehensive portfolio of antibodies, antigen pairs, and ancillary reagents from target discovery through clinical validation. With expertise in bone biology and neurodegenerative disease markers, we support your assay development with validated antibodies like anti-TMEM119, optimized for WB and ELISA. Let CamelBio streamline your sourcing of rare-target raw materials, ensuring consistent supply and performance for diagnostic manufacturing.