Anti-TMEM119 Monoclonal Antibody for WB, IF-P, IHC-P, mIHC, ELISA - Q4V9L6

Core Product Specifications and Parameters

Biological Background: TMEM119 Function and Localization

• Transmembrane protein 119 (TMEM119), also known as osteoblast induction factor (OBIF), is a single-pass type II transmembrane protein involved in bone formation and normal bone mineralization (PubMed:20025746). Related references: PMID:20025746
• It promotes the differentiation of myoblasts into osteoblasts through enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway, and up-regulates ATF4, a key transcription factor in osteoblast differentiation.
• TMEM119 is essential for normal spermatogenesis and late testicular differentiation, as shown by similarity studies.
• Subcellularly, it localizes to the cell membrane, cytoplasm, endoplasmic reticulum membrane, and is also secreted.
• In tissues, TMEM119 is expressed in brain microglia (at protein level) (PubMed:26250788), with elevated levels in Alzheimer disease brains. It is also detected in urine (PubMed:37453717) and expressed by osteoblast-like cells in bone and follicular dendritic cells in lymphoid tissues.
• The protein is classified under keywords including biomineralization, osteogenesis, glycoprotein, and spermatogenesis, highlighting its role in bone and reproductive biology.

Experimental Guidance and Technical Tips

• For Western blotting, validate the antibody in a relevant cell line or tissue expressing TMEM119; the predicted molecular weight is approximately 29 kDa. Consider using whole-cell lysates from osteoblast-like cells or brain microglia.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), optimize antigen retrieval conditions and test on formalin-fixed paraffin-embedded human brain or bone tissue sections. Co-staining with established microglia (e.g., IBA1) or osteoblast markers can confirm specificity.
• In multiplex immunohistochemistry (mIHC), verify compatibility with other primary antibodies and detection systems to avoid cross-reactivity.
• The antibody's cross-reactivity with mouse and rat makes it suitable for rodent model studies, but always confirm reactivity in your specific experimental system.
• As the immunogen is a recombinant protein covering full-length human TMEM119, epitope accessibility may vary across applications; titrate the antibody for optimal performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, from antibodies and antigens to ancillary reagents. This TMEM119 monoclonal antibody is suited for bone biology, microglia research, and reproductive health applications—areas where CamelBio can also provide additional tools such as BMP pathway antibodies, microglia-specific markers, and recombinant proteins. Whether you need validated antibody pairs, bulk quantities, or rare-target reagents, CamelBio streamlines your sourcing from discovery to diagnostic development.