Anti-TMEM100 Rabbit Polyclonal Antibody for WB and ELISA - Q9NV29

Core Product Specifications and Parameters

Biological Background: TMEM100 Function and Localization

• TMEM100 (Transmembrane protein 100) is a 14 kDa multi-pass membrane protein that contains two predicted transmembrane helices, localizing primarily to the cell membrane, perinuclear cytoplasm, endoplasmic reticulum, and perikaryon in neurons.
• During embryonic development, TMEM100 plays a critical role in arterial endothelium differentiation and vascular morphogenesis by participating in the ACVRL1 receptor-dependent signaling pathway activated by bone morphogenetic proteins such as GDF2/BMP9 and BMP10.
• In the peripheral nervous system, TMEM100 regulates nociception by modulating the interaction between the pain sensors TRPA1 and TRPV1 in dorsal root ganglia neurons; it weakens TRPA1-TRPV1 association, alleviating TRPV1-mediated inhibition of TRPA1 and enhancing channel activity.
• Consistent with its vascular and neuronal functions, TMEM100 is expressed in arterial endothelial cells (e.g., umbilical artery) and in neurons of the central nervous system, peripheral nervous system, and enteric plexuses (gastric body, jejunum, proximal colon).
• Keywords from UniProt highlight its involvement in developmental processes and cell differentiation, and it is classified as a phosphoprotein, suggesting regulation by post-translational modification.

Experimental Guidance and Technical Tips

• For WB applications, consider using whole-cell or membrane-enriched lysates from human or mouse tissues expressing TMEM100 (e.g., vascular endothelial cells, dorsal root ganglia). The predicted molecular weight is approximately 14 kDa; a band near this size is expected under reducing conditions.
• For ELISA, the immunogen is a synthetic peptide corresponding to amino acids 50–C-terminus; this peptide may serve as a positive control to validate assay specificity.
• Since the antibody is a polyclonal, batch-to-batch variability is possible; perform appropriate validation using positive and negative controls in the relevant sample system.
• Cross-reactivity is reported for human and mouse; verify reactivity with other species if needed by sequence alignment of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. This TMEM100 polyclonal antibody supports research in vascular development and pain signaling, and we can assist with sourcing related rare-target raw materials tailored to your assay development needs. From concept to clinic, CamelBio offers the technical services and supply-chain expertise to accelerate your diagnostic projects.