Anti-TMEFF2 Monoclonal Antibody for WB, ELISA - Q9UIK5

Core Product Specifications and Parameters

Biological Background: TMEFF2 Function and Localization

• Tomoregulin-2 (TMEFF2), also known as hyperplastic polyposis protein 1 (HPP1) and transmembrane protein with EGF-like and two follistatin-like domains, is a single-pass transmembrane protein encoded by the TMEFF2 gene; alternative splicing produces multiple isoforms, with isoform 3 expressed in testis and prostate.
• It contains an EGF-like domain and two follistatin-like domains, classified as a proteoglycan with disulfide bonds and glycosylation sites.
• The protein functions as a survival factor for hippocampal and mesencephalic neurons; its shedded extracellular form can up-regulate cancer cell proliferation, likely via promoting ERK1/2 phosphorylation.
• Subcellularly, TMEFF2 localizes to the membrane and is also secreted; it is highly expressed in adult and fetal brain, spinal cord, and prostate, with highest levels in amygdala and corpus callosum, and is found in the pericryptal myofibroblasts of normal colonic mucosa.
• Expression is markedly down-regulated in colorectal cancer, while it is detected in prostate carcinoma; at the protein level, it is present in Alzheimer disease plaques, suggesting roles in both neuroprotection and tumor biology.
• The protein undergoes alternative splicing and is proteolytically processed, generating membrane-bound and secreted forms that regulate extracellular signaling.
• TMEFF2 is annotated as a glycoprotein and proteoglycan, containing a signal peptide, a transmembrane helix, and two follistatin-like repeats, reflecting its multifaceted roles in development and disease.

Experimental Guidance and Technical Tips

• For Western blot analysis, detect TMEFF2 at the predicted molecular weight of 41 kDa; validate band specificity using TMEFF2-expressing cell lines such as prostate cancer or neuronal models.
• When performing ELISA, optimize antigen coating concentrations and antibody dilutions according to your assay conditions; consider pairing with a validated detection antibody for sandwich formats.
• Given reported cross-reactivity with rat, this antibody may be suitable for studies in rat brain or spinal cord tissues; always confirm reactivity in your specific experimental system.
• The immunogen targets amino acids 275–374, which lies within a region shared by major isoforms; verify isoform-specific detection if required for your study.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials for neuroscience and oncology biomarker development. We supply validated antibody pairs, optimized monoclonal antibodies like this TMEFF2 clone, and bulk ancillary reagents to streamline your assay development. For rare targets or custom sourcing needs, CamelBio bridges the gap from concept to clinic with dedicated technical support.