Anti-TMED3 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y3Q3

Core Product Specifications and Parameters

Biological Background: TMED3 Function and Localization

• TMED3 (Transmembrane emp24 domain-containing protein 3), also known as p24B, p24gamma4, p26, and C15orf22, is a member of the p24 family of trafficking proteins.
• It potentially plays a role in vesicular protein trafficking, primarily within the early secretory pathway.
• TMED3 contributes to the coupled localization of TMED2 and TMED10 at the cis-Golgi network, suggesting a role in maintaining organelle organization.
• Subcellularly, TMED3 localizes to multiple membrane compartments including the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment (ERGIC), cis-Golgi network, and Golgi stack membranes.
• It is also found in cytoplasmic vesicles, particularly COPI-coated vesicles, consistent with its function in retrograde and anterograde transport.
• The protein contains a signal peptide, a transmembrane helix, and is subject to alternative splicing.
• Keywords: Alternative splicing, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane, Protein transport, Proteomics identification, Reference proteome, Signal, Transmembrane, Transmembrane helix, Transport.

Experimental Guidance and Technical Tips

• The polyclonal antibody is raised against a recombinant fragment corresponding to amino acids 24-180, which encompasses the luminal domain of TMED3. This region may offer good target specificity.
• For Western blot, a band around 25 kDa is expected; consider using reducing conditions and validate in relevant human, mouse, or rat tissue/cell lysates.
• Immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC) may detect TMED3 in the ER-Golgi intermediate compartment; optimize antigen retrieval and permeabilization protocols for best results.
• ELISA applications can be used for quantitative detection; a standard sandwich ELISA setup with appropriate controls is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing solution for diagnostic manufacturers and researchers, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For targets like TMED3, involved in the early secretory pathway and organelle dynamics, CamelBio ensures reliable access to high-quality raw materials to support your assay development and research needs. Our integrated supply network helps accelerate your projects from concept to clinic.