Anti-TMED2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q15363

Core Product Specifications and Parameters

Biological Background: TMED2 Function and Localization

• TMED2, also known as p24, p24beta1, or RNP24, is a member of the p24 family of transmembrane proteins that function in vesicular trafficking.
• It acts as a cargo receptor in the early secretory pathway, specifically for GPI-anchored proteins in COPII vesicles, in partnership with TMED10.
• TMED2 also participates in COPI-mediated retrograde transport by inhibiting ARFGAP1 activity, regulating vesicle uncoating.
• It facilitates the exocytic trafficking of certain G protein-coupled receptors (GPCRs) such as F2RL1, OPRM1, and P2RY4 from the Golgi to the plasma membrane.
• Subcellularly, TMED2 localizes to the endoplasmic reticulum, ER-Golgi intermediate compartment, Golgi cisternae, and COPI-coated vesicles.
• The protein contains a signal peptide, a coiled-coil domain, and a single transmembrane helix, and is involved in the maintenance of Golgi architecture.
• It may also play a role in the biosynthesis and processing of secreted cargo.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the lumenal domain (a.a. 60-170) of human TMED2, a region conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For western blot (WB), the predicted molecular weight is ~23 kDa; however, post-translational modifications such as N-glycosylation may result in a higher apparent band.
• For immunofluorescence (IF/ICC), permeabilization is required due to TMED2's membrane association; consider co-staining with organelle markers to confirm localization.
• For ELISA, the antibody can be used for antigen detection; optimal working concentration should be determined experimentally.
• Always include appropriate positive and negative controls (e.g., TMED2-overexpressing and knockout cell lines) to validate specificity in your experimental system.
• These are suggestions; actual performance may vary depending on experimental conditions and should be verified by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive suite of IVD raw materials and technical support for research and diagnostic development, from concept to clinic. This anti-TMED2 polyclonal antibody exemplifies our expertise in providing high-quality antibodies for studying vesicular trafficking and cargo receptor biology. Explore our catalog for validated antibody pairs, bulk reagents, and custom sourcing solutions for rare targets to accelerate your projects.