Anti-TMC1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8TDI8

Core Product Specifications and Parameters

Biological Background: TMC1 Function and Localization

• TMC1 is a pore-forming subunit of the mechanotransducer (MET) non-selective cation channel complex, essential for auditory sensory transduction.
• The MET channel is located at the tips of stereocilia of cochlear hair cells and is activated by tension in the tip-link extending from one stereocilium to the adjacent shorter stereocilium.
• TMC1 forms dimeric ion-conducting subunits within the MET complex, aided by auxiliary proteins LHFPL5, TMIE, CIB2/3, TOMT, and PCDH15.
• TMC1 MET channel is highly permeable to calcium and likely transports monovalent cations (By similarity).
• It is also involved in vestibular hair cells transduction current (By similarity).
• Subcellularly, TMC1 localizes to the cell membrane.
• Post-translational modifications: TMC1 is classified as a phosphoprotein.
• Tissue specificity: Detected in fetal cochlea and at low levels in placenta and testis.

Experimental Guidance and Technical Tips

• As a polyclonal antibody raised against a peptide immunogen (residues 600-700), it is expected to detect both native and denatured TMC1 protein, making it suitable for Western blotting (WB).
• For WB, validate the antibody in your sample system and consider loading controls and molecular weight markers to confirm specificity at ~88 kDa.
• For IHC-P, antigen retrieval protocols may be required; optimization with positive control tissue (e.g., cochlea) is advised.
• For ELISA, the antibody can be used for capture or detection after proper pairing validation.
• Cross-reactivity with mouse and rat TMC1 is indicated; sequence alignment of the immunogen region is recommended to confirm epitope conservation.
• Note that TMC1 expression is tissue-specific; ensure relevant positive controls are included.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined procurement path for high-quality IVD raw materials like this anti-TMC1 antibody. Whether you need validated antibody pairs for auditory mechanotransduction assays, bulk polyclonal antibodies, or ancillary reagents, our catalog and sourcing network accelerate your development from concept to clinic. Our team also offers technical consulting to support rare target assays.